Analyzing Oct4 expression level in PBMC of multiple sclerosis patients

Abstract

Multiple sclerosis (MS) is an autoimmune disease with inflammatory and demyelinating lesions in the nervous system, characterized by lymphocyte infiltration and damage to axons. In some studies conducted in recent years, the role of OCT4 in the regeneration of nerve myelin in patients with MS has been mentioned using experimental models and oligodendrocytes, which suggests a new therapeutic strategy for MS by restoring myelin with the help of this gene. Have. The aim of this study is to investigate the expression level of OCT4 gene in PBMC of MS patients. Methods: In this study, first of all, 40 patients referred to the hospital, in whom multiple sclerosis was diagnosed by a specialist based on diagnostic criteria, and 40 control group without autoimmune disease, which is 5% of all participants. Thirty blood samples were taken and PBMC was isolated and participants' RNA was extracted, then the cDNA synthesis step was performed, and then the expression level of OCT4 was determined by real time-PCR using the Cybergreen method. The difference between the variables between the groups was checked with proportional statistical tests. In statistical tests, p-value < 0.05 was considered significant. Results: The average age in the control group was 37.55 ± 7.14 years and in the MS group was 40.60 ± 10.54 years. In the control group, 34 participants (85%) and in the MS group, 10 participants (2.5%) were female. In the MS group, 33 (82.5%) of the patients had relapsing-remitting (RR) type of MS disease. All the control subjects were negative for FH, and in the MS group, 9 people (22.5%) were positive for FH. The results showed that the difference in mean expression of OCT4 in healthy and sick female participants is significant (P=0.016). This statistical analysis showed a statistically significant difference in the expression of this gene in women with MS and healthy men (P=0.000). The average expression level of this gene in control subjects and in the group with MS, at ages less than 40 years and more than 40 years, this difference was statistically significant. The statistical difference between the control group and the group of patients with MS with RR type was significant. Also, the sensitivity of OCT4 for the diagnosis of MS was 75% and its specificity was 60.98% (P>0.0001), which indicates the diagnostic value of this gene in MS.