Investigating the genetic diversity of aquatic bacteria of lake urmia in estuary of jighati (zarrine rood) and tatao (simine rood) rivers in the fall of 1400(2021)
چکیده
Introduction: Lake Urmia is one of the saltiest lakes in the world, which faces the risk of dryness and excessive salinity. Aquatic microorganisms play an important role in the decomposition of substances, food chains and biochemical cycles and can also have functional effects. Therefore, the isolation and identification of bacteria is very necessary to better understand their function in an ecosystem like Urmia Lake, which is constantly changing.Objectives: The objectives of this study are to isolate and purify the bacteria in the water collected from the river mouth of Zarinerood and Siminerood rivers, and then to determine the 16sRNA sequence of the isolated bacteria to determine the identity of the bacteria and also to investigate the biochemical properties of gram, catalase, oxidase, and and motility of bacteria.Methodology: The population of bacteria was cultured in the form of single colonies during the growth process and successive isolations. After the DNA extraction stage and if the quality of the results of qualitative and quantitative investigation was suitable, the selected bacteria were sent for sequencing and also phenotypic tests were performed on the isolated bacteria.Findings: In this study, bacteria isolated from Lake Urmia were isolated, and finally 9 bacterial isolates were subjected to phenotypic studies. According to the results, the majority of these bacteria were gram positive, catalase positive, KOH negative, oxidase positive and amylase negative. DNA extracted from these bacteria was sent to determine the sequence of 16srRNA. The results of this study indicate the possibility of reducing the types of bacteria in this place.conclusion: According to the obtained results, the phenotypic results also do not show much diversity among the selected species and there is a possibility of reducing the bacterial species in this place.