Evaluating of miR-10a expression and its relation with regulatory T lymphocytes frequency and TGF-β and IL-10 levels in Multiple sclerosis patients compared to healthy controls

Abstract

MS (multiple sclerosis) is the most common autoimmune disease of the central nervous system, which was first described in 1866 by Charcot and Vulpain. Multiple sclerosis is an inflammatory demyelinating disease of the central nervous system, which is associated with inflammatory infiltrates, progressive demyelination of axons, and finally axonal loss. Given the description of miRNAs and miR-10a and its actions are well conserved among vertebrates, it is recognized as an important post-transcriptional mediator in the control of inflammation. Considering that miR-10a is one of the modulators of immune system activity and in many diseases such as autoimmune diseases, as well as cancer, the role of this molecule has been reported as a molecule that increases the activity of regulatory T lymphocytes, the study of pathways The effect and regulation of this miRNA seems to be useful in order to find new therapeutic ways for patients with MS.

Materials and methods: This study was done in a descriptive-analytical way. Written and informed consent was obtained from 40 patients with MS referred to Razi Medical Center in Tabriz and 40 healthy individuals who are our study populations in this research. Then after taking blood from people, peripheral blood mononuclear cells were separated using Ficoll and density gradient centrifugation and the abundance of Treg cells and gene expression were measured using flow cytometry technique. Then, data distribution was checked using prism software and Test-T test was used for normal distribution and Whitney-Mann test was used for data with non-normal distribution.

Results: The results of the study showed that the level of expression and secretion of TGF-B and IL-10 cytokines in patients is significantly reduced compared to healthy people. Also, miR-10 gene expression is decreased in patients. The results of the relationship between the expression of miR-10 and cytokines related to Treg also showed that this relationship between miR-10 and the frequency of Treg cells and related cytokines is directly significant.