Investigating the effect of decreasing SLC16A13 gene expression on apoptosis and metastasis in lung cancer cell line

Abstract

Lung cancer is one of the most common cancers in the world with avery high mortality rate that has different pathological subcategories,among which NSCLC is the most common form of this malignancy. The complex pathogenesis of this disease has caused the treatment of this disease in advanced stages to be accompanied by many problems. Recently, the genes involved in metabolism, especially those coding for membrane transporter proteins (the solute carrier) have been noticed in cancer. Due to their important role in the homeostasis of cells, they have been noticed as possible targets of treatment in various malignancies, especially lung. SLC16A is a member of the family of membrane transporters whose importance in the promotion of cancer has been revealed in recent years. In the present study, we tried to knock down the expression of SLC16A-13 gene in lung cancer cell line (A549) to investigate the effect of reducing the expression of this gene on the ability of survival, growth and metastasis in these cells..

Matherial ans methods: First, lung cancer cell lines (A549) were cultured in standard medium and then transfected into the target cells to reduce the expression of the target gene, synthetic si-RNA of SLC16A13 antigen. Then, we used MTT and flow cytometry tests to investigate the effect of reducing the expression of the studied gene on the process of cell death and apoptosis. SCRATCH test was also used to investigate the role of this treatment on the migration ability of cancer cells. Also, the effect of this treatment on the change of target gene expression will be analyzed by western blot test and Real Time PCR. Finally, the expression changes of genes involved in apoptosis and cell migration were evaluated by Real Time PCR method. Result: According to the results obtained from the present study, the reduction of SLC16A13 gene expression causes an increase in the apoptosis ratein lung cancer cells, but does not change the ability of cancer cells to migrate.