In vitro survey of Extract of Eucalyptus Microtheca on Acanthamoeba

Abstract

The Acanthamoeba genus is free-living amoebae and opportunistic pathogens that cause amoebic keratitis and granulomatous encephalitis. Common therapeutic drugs have severe toxicity. Therefore, the World Health Organization emphasizes the comprehensive approach of traditional medicine and medicinal plants. One of these plant compounds is the Eucalyptus microtheca leaf, the leaf extract of this plant, like most plants, has anti-parasitic properties. This study aim to determine the effect of the aqueous extract of the Eucalyptus microtheca plant against trophozoites and Acanthamoeba cysts in vitro. Method In this experimental study the Acanthamoeba sample cultured in a non-nutritive agar culture medium, and then the aqueous extract of Eucalyptus Microtheca was prepared. (30-60-90-120 minutes) in concentrations (10-20-40-80 mg/ml) .0.02% chlorhexidine and PBS were used as a positive and negative controls and the amount We examined its viability with 1% eosin dye Result The aqueous extract of Eucalyptus Microtheca significantly reduced the average number of trophozoites and parasite cysts in terms of time and concentration, so that after 24 hours at a concentration of 80 mg/ml, 100% of the cysts were destroyed and in 120 minutes In the said concentration, 0.67% of trophozoites survived