Comparison of the effect of different bleaching methods on teeth discolored by two types of antibiotics used in revascularization protocols.

Abstract

Introduction Regenerative endodontic procedure (REP) are known as a new approach in the root treatment of immature permanent teeth, which allow the growth and development of the immature roots of necrotic permanent teeth. One of the main factors in the success of these treatments is adequate cleaning and disinfection of the root canal of immature teeth. For this purpose, the American Association of Endodontists (AAE) has introduced the triple antibiotic paste (TAP), which includes the antibiotics minocycline, ciprofloxacin and metronidazole. Although its many advantages, this combination has disadvantages such as changing the color of the teeth,. Therefore, it is useful to correct the color of the discolored teeth with conservative methods, one of these methods is Bleaching treatment. The purpose of this study is to compare different Bleaching methods to correct the color of discolored teeth.

Material & Methods First, 60 newly extracted anterior teeth, which are single canal and single root, were collected and after disinfection with 1.5% sodium hypochlorite for 3 minutes, in order to simulate the immature apex, changes were made on the teeth and the length of all The teeth were considered to be 15 mm on average. Wax was placed at the ends of the cut roots. Then, after creating the access cavity in the teeth and completely cleaning the canals and finally the access cavities were temporarily repaired. Then the samples were randomly divided into two groups of 30 and inside the root canal of the teeth, up to the level of the CEJ, two types of antibiotics generally used in endo revascularization treatments, namely TAP 1 (ciprofloxacin, metronidazole, amoxicillin) and TAP 2 (ciprofloxacin, metronidazole, minocycline) were placed separately. Then the samples were temporarily restored with temporary restorative materials and in the next step, the changes in the color of the teeth in weeks 0 (immediately after contact with antibiotics), 3 and 6 separately They were analyzed and compared with a spectrophotometer. After 6 weeks have passed since the last analysis of color changes caused by antibiotics, in the next step, the teeth of each group were randomly divided into three subgroups, and

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the teeth of each subgroup were treated with different methods and protocols with three commonly used substances in Teeth bleaching, sodium perborate, hydrogen peroxide, carbamide peroxide were bleached. The color analysis of all samples was done separately in weeks 0 (immediately after bleaching), 3 and 6 with the help of a spectrophotometric device. To compare the effect of time and color changes in teeth after contact with antibiotics and bleaching materials, from the test Two Factor repeated mesure (ANOVA) and SPSS22 statistical software were used. Results The amount of teeth color changes in the first group (TAP1) was less than the second group and also various bleaching methods were effective in correcting the color of discolored teeth, but there was no significant difference between the types of methods. Conclusions Triple Antibiotic Paste (TAP) changes the color of teeth, and TAP with minocycline caused more color changes in teeth than TAP with amoxicillin, and also bleaching can partially correct the color of discolored teeth.