Study of changes in expression pattern of endothelial-mesenchymal transition genes (EndMT) of endothelial cell model treated with

Abstract

Cancer is one of the leading causes of illness and death worldwide. Tumor cells grow in a complex microenvironment consisting of immune, stromal, and vascular cells that support growth, angiogenesis, and metastasis. Endothelial cells (ECs) are the major components of the vascular environment. These cells have been described for their plasticity and their potential for transmission to mesenchymal cells through a process known as endothelial-to-mesenchymal transition (EndMT). Cordycepin, a substance in traditional Chinese medicine, shows several pharmacological mechanisms in the treatment of tumors, but its mechanisms have not been fully elucidated. In this study, HUVEC cells were treated with Cordycepin and after 24 hours, the total RNA of the cells was extracted and cDNA was synthesized. The difference in expression of CD31, VE cadherin, vimentin, a-SMA genes in treated and untreated cells will be investigated by real-time PCR. Methods: HUVEC cell line was cultured in complete RPMI-1640. Cells were treated for 24 hours with DTX, CoCl2, DTX and CoCl2. Cells were harvested, and RNA extraction and cDNA synthesis were performed from cells and cordycepin using standard methods. Expression levels of EndMT genes were analyzed using quantitative real-time PCR (qRT-PCR). Results: Our examination has showed that Cordycepin has no significant effect on the inhibition of endothelial to mesenchymal transition in the tumor micro environment