Increase of central tolerance towards skin graft rejection antigens in mouse model

Abstract

Skin grafting is a model of grafting done on the skin, which is done due to factors that come out of the natural tissue. Skin grafts are commonly used to treat major trauma and burns. In certain surgeries that require skin grafts for treatment, such as skin cancer, some skin is removed. Parts of the skin that require skin grafting: If the burn is extensive, the priority for grafting is the face, neck, hands, elbows, armpits, and knees to avoid deformity and skin shrinkage. In choosing the skin for transplantation, the priority is the person's own skin, but sometimes it may be used for reasons such as: the impossibility of repairing one's own skin, the need for another person's skin for repair. materials and methods: First, the number of 84 adult male and female three-week-old laboratory mice of the Balb/c breed and 4 male and three-week-old female mice of the DBA breed, weighing 280-300 grams, will be prepared and used from the animal breeding and maintenance center of Tabriz University of Medical Sciences. This study has 5 phases, in the first phase, it was conducted with the aim of the appearance of the antigen that causes transplant rejection on the APCs of the transplant recipient mice, during which cells from the skin of the transplant donor mice (4 Balb/c mice and 4 DBA mice) Done. which will be transplanted. In the second phase, which is done with the aim of the emergence of T cells with high tolerance to transplant rejection genes in the circulation, the immunosuppressor drug, cyclosporine A, is started orally at a dose of 10 mg/kg and its concentration increases to 64 mg/dl. In the third phase, donor and recipient mice will be checked for HLA matching.In the fourth phase, if the initial transplant is successful, from one Balb/c mouse to 40 Balb/c mice (20 mice received the immunosuppressor drug groupAnd finally, in the fifth phase, after 40 mice receiving the first and second transplants and APC, the number of T cells in the circulation reached a normal value and confirmed the success of the first transplant. Results:The results of transplantation showed the appearance of transplant rejection gene in APC of transplanted mice. Also, in this study, T cells with high tolerance to transplanted genes appeared in the bloodstream. There was HLA compatibility between donor and recipient mice, and tolerance of B cells against non-self antibodies was formed in skin grafts. The effectiveness of the first transplant and its comparison with the effect of immunosuppres after transplant was not significant. Conclusion: According to the present study, the results showed that weakening in skin grafting alone was not enough for the role of grafting, and after transplantation, our need for immunosuppressive drugs would be reduced.