Association of CD39 with ENTPD1, ZEB1, ZEB2 genes pancreatic cancer

Abstract

Today, cancer is one of the most important issues for researchers, finding a solution for early diagnosis or timely suppression of cancer in the tissue is of particular importance. For this purpose, in this research, we investigated the genes involved in pancreatic cancer (ZEB1, ZEB2, CD39) and the relationship between CD39 expression changes and ZEB1, ZEB2 expression changes. Method: In the samples collected and immediately after the pancreatic cancer surgery, we transferred to nitrogen tanks and stored at -70 temperatures. The extraction was done manually using Trizol. It was done and after examining the OD, extracted RNA, cDNA synthesis was done and then the expression level of ZEB1, ZEB2, CD39 genes was measured by Real-time PCR technique and for statistical comparison of gene expression results, depending on the type of data distribution (normal or non-normal) Student t test or Mann-Whitney U statistical tests were used. Result: The results of Real-time PCR showed that all three genes, ZEB1, ZEB2, CD39 had increased expression in pancreatic cancer tissue compared to normal tissue. On the other hand, it seems that increasing the expression of CD39 increases the expression of both ZEB1 and ZEB2 genes.