Molecular typing of Multi Drug Resistant Klebsiella pneumoniae isolated from hospitalized patients by the MLVA methods at Tabriz during 2020

Abstract

Klebsiella pneumoniae is one of the most important opportunistic pathogens and is an important cause of hospital infections. The prevalence of antibiotic resistance of Klebsiella pneumoniae is increasing worldwide and can cause many clinical problems. Therefore, the purpose of this study was to determine the frequency of resistant strains, as well as the molecular typing of strains from Tabriz hospital samples. Methods: In this study, 100 samples of Klebsiella pneumoniae were collected from different samples (blood, urine, wounds and respiratory secreation) of inpatients in Imam Reza Hospital, Tabriz. After the final confirmation with biochemical tests, the antibiotic sensitivity test was performed. Typing of Klebsiella pneumoniae strains was done by MLVA method. Result: Eighty-six multidrug-resistant (MDR) K. pneumoniae isolates were identified, which resistance to ampicillin, trimethoprim/sulfamethoxazole, and ceftriaxone was the most frequent in the considered isolates (100%, 93%, and 93%, respectively). A total of 50 different antibiotic susceptibility patterns were observed among the MDR K. pneumonia. After typing isolates by MLVA method, 5 clusters were identified. The frequency of MDR isolates in the present study was 86%. After typing MDR isolates by MLVA method, 37 types were identified, which were placed in 5 clusters A to E.