Evaluation of MEIS1 gene expression in HIRA / MEIS1 / Myocardin pathway in patients with premature coronary artery disease and the relationship of this pathway with inflammatory factors

Abstract

Coronary artery disease (CAD) that manifests in young adults can have devastating consequences. In addition, the study of young patients with CAD may provide insight into the genetic basis of coronary disease. It is estimated that significantly less than 10% of people with coronary artery disease are younger than 40 years old. However, this group of patients may be disproportionately significant because CAD presenting in young adults can lead to devastating outcomes for these patients, their families, and society. Meis1 plays an important role in vascular network development and heart development. Also, this gene has a great effect on the regeneration capacity of the heart. The purpose of this study is to investigate the expression level of MEIS1, HIRA and Myocardin genes in people with premature coronary artery disease and to investigate the relationship between these genes and possible inflammatory factors in these patients.

Materials and Method

In this case-control study, after approval of the ethics committee, a total of 35 patients with a diagnosis of coronary artery disease and 35 controls presenting to Madani hospital during 1402 will be included through simple randomization. Written informed consent will be obtained from all the participants. Patients with inflammatory disease, metabolic syndrome, renal or hepatic failure, severe or moderate valvular dysfunction will be excluded. 5 cc blood sample will be taken from the patients in order to evaluate for inflammatory genes including IL-6, IL-10 and TNF alpha as well as HIRA, MEIS1, and cardiolipin genes via extracting RNA from mononuclear cells real-time PCR was performed. Data will be analyzed using IBM SPSS 24. Data with normal distribution will be analyzed through Independent T-test and data without normal distribution through the Mann-Whitney U test. P less than 0.05 will be considered as significant.

Results:

Our research has demonstrated a distinct disparity in the expression levels of Meis1, HIRa and Myocardin as early myocardial marker genes between individuals with CAD and those in a normal physiological state. Additionally, it appears that two specific genes, Meis1 and HIRA, may play a significant role in regulating the synthesis of pro-inflammatory cytokines, namely TNF-a and IL-6. Interestingly, the study suggests that Meis1 and HIRA may also be involved in suppressing the production of an anti-inflammatory cytokine called IL-10.