Evaluation of TG (rs853326) and CTLA4(rs231779) gene polymorphisms in individuals with anti-TPO-positive serum levels and healthy individuals with normal Anti-TPO: a case-control study

Abstract

Considering the high prevalence of thyroid diseases, especially autoimmune thyroid diseases, identifying genotypes and related polymorphisms for screenings and other diagnostic measures is very important. It can also be a basis for further studies. This study was conducted with the aim of investigating the sequence polymorphism of TG genes (rs853326 and CTLA4 gene (rs231779) in people with positive Anti-TPO serum levels with symptomatic disease and healthy people with positive Anti-TPO levels who had serum levels of vitamin D less than 28 are. Methods: In this case control study, 82 Anti-TPO positive patients (mean age 41.48 ± 9.58 years) and 82 people with Anti-TPO positive without symptomatic disease were included in the study, while they were selected from people with vitamin D less than 28. . Then the serum levels of vitamin D and TSH were measured and based on the level of TSH, they were divided into two categories: hypothyroid and euthyroid. After blood sampling and DNA extraction, polymorphisms of TG gene (rs853326) and CTLA4 gene (rs231779) were investigated using tetra-ARMs PCR technique. Results: The frequency difference of rs853326 genotypes including AA, AG and GG between hypothyroid and euthyroid groups was not significant (P>0.05). In the examination of CC genotype (rs231779) considering that the frequency of TT genotype in both groups is zero and the frequency of CC and CT genotypes in both groups is equal, so the P-value obtained for both genotypes will be equal to 1 and There is no significant difference in the hypothyroid and euthyroid groups, but in Hardy-Weinberg equilibrium, the p-value of the rs231779 position is less than 0.05, so the difference observed for the observed and expected genotypes between the two groups is significant, and the population is out of balance. Conclusion: In the study of rs231779 SNP, For GG and AG and AA genotypes, there is no relationship between healthy and diseased groups, but there is a significant relationship between rs853326 and Hardy-Weinberg equilibrium ..

In the study of rs231779 SNP, the frequency of TT genotype in both groups is zero, and the frequency of CC and CT genotypes is equal in both groups, so there is no significant relationship between these genotypes and the two healthy and sick groups. (It should be noted that because this study is part of a larger study on the relationship between vitamin D deficiency and autoimmune diseases, in this study it was conducted on people whose vitamin D level was less than 28 units.).