Comparison of free and PLGA-PAA nano-encapsulated of HydroxytyROSol and Doxorubicin effects on hTERT and CREB gene expression in MCF7 breast cancer cell line

Abstract

Breast cancer is the most commonly diagnosed cancer (24.5% of all cancers) and the leading cause of cancer death (15.5% of all cancer deaths) in women worldwide in The year has been 2020.The primary objective of this research is to assess the impact of HydroxytyROSol and Doxorubicin on hTERT and CREB gene expression in MCF-7 breast cancer cell line by comparing the effects of free and PLGA-PAA nano-encapsulated forms.

Literature review: Mediterranean countries known for their consumption of vegetables and olive oil have a lower incidence of breast cancer, possibly due to the positive effects of phenols such as hydroxytyROSol found in olive oil. New cancer treatment methods use the nanoencapsulation process and combination with chemotherapy drugs to improve the drug's performance.

Method and material: PLGA-PAA nanoparticles have been successfully synthesized. The MCF7 cell line has been cultured in a cell culture medium. In order to investigate the anti-proliferative and anti-tumor effects, the FREE and nano-encapsulated forms of HydroxytyROSol and doxorubicin, as well as their combination, have been examined using the MTT assay. Furthermore, total RNA has been extracted from both the control and treated groups, followed by the synthesis of cDNA using the Reverse transcriptase enzyme. Subsequently, real-time PCR has been conducted to analyze the data. By comparing the real-time PCR data between the control and treated groups, the expression differences in the seven studied groups have been identified. Results: The results of the MTT test indicated that IC50 for hydroxytyROSol capsule was 9.5 ppm and the combination of two drug capsules was 5.3 ppm, which is lower than their free form. Real-time PCR results revealed that in the three groups receiving the nanocapsulated drugs, the expression levels of hTERT, CREB1, and CREB2 genes in the MCF7 cell line were comparatively reduced in relation to the free form.