Molecular analysis of TNFAIP3 (Rs2230926, Rs5029937) gene polymorphisms in patients with palindromic rheumatism

Abstract

Objective: Palindromic rheumatism is characterized by frequent and irregular attacks without fever, arthritis, periarthritis, and sometimes swelling and erythema of the soft tissue around the joint, which occur at unspecified intervals and last for several hours to several days. Tumor necrosis factor-induced protein (TNFAIP3) plays an important role in the pathogenesis of autoimmune diseases. The present study aimed to investigate the polymorphisms (Rs2230926, Rs5029937) of the TNFAIP3 gene in patients with palindromic rheumatism and RA patients. has been Method: The total volume of the studied sample was 120 people. These people were selected from the patients referred to the hospital who had palindromic rheumatism diagnosed by a rheumatologist based on diagnostic criteria, and 40 patients with rheumatoid arthritis and 40 control group without autoimmune disease were selected. After blood collection and RNA extraction, Rs2230926 and Rs5029937 polymorphisms were investigated using tetra-ARMs PCR technique. Results: Hardy-Weinberg equilibrium analysis of Rs5029937 position for GG, AG and AA genotypes and Rs2230926 position for TT, TC and CC genotypes for RA patients showed that the difference observed for the observed and expected genotypes between the two groups was significant, but for the observed and expected genotypes of both loci between the two control groups and patients with palindromic rheumatism, it was not significant. Among the genotypes of the Rs2230926 locus including TT, TC and CC, the frequency of the TT genotype was significantly different between the control groups and the group of patients with palindromic rheumatism (P=0.026), also the calculated OR is significant, so that the TT genotype It reduces the risk of disease by 0.138 times. Examining the allelic frequency and their significance for the Rs2230926 position between the control group and palindromic rheumatism showed that the allelic frequency between the two groups is significant, and according to the OR value of the C allele compared to the T allele, the risk of contracting the disease is 232/ increases 2 times (Odds Ratio=2.232).