Utilizing the aerogel nanocomposite for extraction and determination of metoprolol in the biological samples

Abstract

Metoprolol (MET) is a selective β-adrenergic antagonist used to treat cardiovascular disarranges such as hypertension, angina pectoris, cardiac arrhythmias, and myocardial localized necrosis. MET decreases heart rate and hand tremors, therefore competitors can mishandle it primarily. In this manner, from the World Anti-Doping Organization and Universal Olympic Committee, its utilization is denied by competitors. On the other hand, overusing MET leads to bradycardia, hypotension, and fatigue. The characteristic significance of β -blockers in doping, forensic science, and toxicological settings and the requirement for ideal measurement alteration in clinical settings has driven analysts to create expository strategies that can be utilized to precisely screen these drugs.Aim:Extraction and determination of MET in exhaled breath condensate by utilization of an NCAs grafted with graphene oxide (GO )- Fe3O4 .Method:An aerogel system modified with graphene oxide-iron nanocomposite (NC) was synthesized. After optimizing all the parameters affecting the extraction, the drug was extracted by the designed system and the fluorescence intensity was measured at 310 nm with an excitation wavelength of 274 nm. Results:In this work, the experimental conditions such as pH, amount of Fe3O4@rGO/agarose aerogel, adsorption/desorption time, and elution conditions were checked and optimized. The calibration curve was linear in the concentration range of 0.005 to 2.0 μg.mL-1 with a detection limit of 0.003 μg.mL−1 for EBC. The correlation coefficient of the curves was 0.9999. The intra-day and inter-day relative standard deviations (RSDs) were obtained 1.68% and 2.22% for 0.01 μg.mL-1, 2.37%, and 3.95% for 0.05 μg.mL-1 and 1.33% and 2.82% for 0.1 μg.mL-1 of MET, respectively. The accuracy of the method according to relative error (ER)) is less than 5%. Conclusion:A simple and effective method for extraction and determination of METin biological samples is presented with proper precision and accuracy.