Evaluation of the functional properties of postbiotics derived from native probiotics isolated from food products in vitro

Abstract

Simultaneously with the increase in consumer awareness and the subsequent increase in research in the field of beneficial natural compounds such as probiotics, prebiotics, synbiotics and postbiotics, today a new concept has been formed in this field, which has created many research challenges. Postbiotics also have the same function as prebiotic compounds and instead of adding new species to the intestinal microbiota, they create probiotics in the digestive system of each person. Therefore, in the present study, we investigate the biological and functional effects of postbiotics derived from indigenous probiotics in exogenous conditions.

Materials and methods: In this study, after the production of cell culture supernatant post-biotics, cell extract and cell cytoplasm extract, antimicrobial effects against Streptococcus mutans and Listeria monocytogenes, antioxidant, antidiabetic and anticancer in different doses determined in the category Colon cancer cells were investigated. In the first stage, the mentioned bacteria are cultured in MRS and YPD culture media and the optical absorption of the culture medium is measured periodically at a wavelength of 600 nm until the absorbance of the culture medium reaches one. In the next step, the standard MIC method was used to check the antimicrobial effect of the produced postbiotics. In this method, while qualitatively examining the antimicrobial effect of the tested compounds, it is possible to calculate the minimum growth inhibitory concentration of the postbiotic compounds against the pathogenic strains of Streptococcus mutans and Listeria monocytogenes. DPPH method was also used in the antioxidant effects section. Next, MTT and flow cytometry techniques were used to investigate the effects of cell viability and apoptosis.

Results: Post-biotics of cell culture supernatant, cell extract and cell cytoplasm extract (PPF), antimicrobial effects against Streptococcus mutans and Listeria monocytogenes, antioxidant, anti-diabetic and anti-cancer in different doses determined in cell lines. Colon cancer was investigated. The results showed that postbiotics significantly decrease the survival of cancer cells and increase the apoptosis of cancer cells (p<0.01).