Study of weight loss effects of separate and combined administration of garcinia cambogia, glucomannan, caffeine and green tea extract in mice

Abstract

Introduction: Obesity is an increasing major problem associated with the emergence of several chronic diseases related to overweight. In the past, extensive research has been conducted on herbal substances to help to weight control. Four substances that have been discussed separately and in combination are caffeine, glucomannan, Garcinia cambogia, and green tea, which have been studied in human and animal exprements. However, until now, no study has been conducted on the combined effects of all four substances on weight loss or weight gain prevention.Objective: Study the effects of weight reduction with combinations of glucomannan, Garcinia cambogia, caffeine, and green tea individually and in combination in NMRI albino mice.Method: Fifty-four NMRI albino mice were divided into 6 groups of 9 mice in each group, and each group placed in 3 cages randomly. The drug compounds were dissolved in 0.4ml of water according to the corresponding group and administered once daily. Weight daily and blood glucose measured every three days, food and water intake were examined during the study. At the end of the 10-day experiment, blood samples were taken from the animals and cholesterol, LDL, HDL, triglycerides, and lipase enzymes were measured.Results: Administering the combination of "Garcinia cambogia, glucomannan, green tea, and caffeine" resulted in significant weight loss in NMRI albino mice compared to administering these substances individually and the control group. The total cholesterol and triglyceride levels in the combination therapy group were lower than in the control group, but there was no difference in LDL and HDL levels between the combination group and the control. Additionally, the lipase enzyme level was highest in the combination group and lowest in the control group among the groups.Conclusion: Administering the combination may lead to better weight control, which requires further research to investigate mechanisms and side effects.