Simultaneous efficacy of Avastin and Garcinia morla extract in anticancer activity on lung cancer cell lines

Abstract

Lung cancer, which is one of the main global causes of cancer-related deaths, is often asymptomatic until advanced stages. When the disease is suspected to be lung cancer, it needs diagnostic measures and staging of the disease. The treatment is also personalized for the patient according to the type of lung cancer and its stage. Lung cancer remains the leading cause of cancer-related deaths, with approximately 1.8 million new cases and 1.6 million deaths per year. The risk increases with age and men are more affected than women. Although smoking is the main cause of this cancer, it can also be related to genetic predisposition. The purpose of this study is to investigate the expression level of factors involved in the metastasis of lung cancer cells (miR-25, PDK1, miR-145 and KLF4) and to investigate the effect of treatment of cells with Avastin and Garcinia Morella plant extract. Materials and methods: For this purpose, after the culture of lung cancer cell lines NCI-H2347, NCI-H522, NCI-H2073 and NCI-H1755 (grades 1, 2, 3 and 4 respectively) and healthy cell line BEAS-2B, the expression level of miR-25 factors, PDK1, miR-145 and KLF4 were evaluated in each of the cell lines. Then the cells were treated with Avastin and Garcinia Morella plant extract alone and in combination. After that, the amount of cell death in each category was measured using the flow cytometry method. Also, the effect of these two substances on the amount of cell migration was evaluated using the scratch test. Real time PCR test was also used to check the expression level of each factor in each cell line. Results: The results of this study showed that, in general, the expression of miR-25 and PDK1 in lung cancer cells increased significantly. On the other hand, the expression of miR-145 and KLF4 was significantly decreased. The results showed that Avastin and Garcinia Morella plant extract alone and in combination increase apoptosis and decrease migration in lung cancer cells. We also measured the effect of these two substances on four factors miR-25, PDK1, miR-145 and KLF4. The results showed that these two substances alone and in combination increase the expression of KLF4 and miR-145