Evaluation of LINC01111, miR-3924 and DUSP1 expression level In tumor samples and healthy margin of pancreatic adenocarcinoma

Abstract

Pancreatic cancer (PC) is caused by the uncontrolled shredding of pancreatic cells, considering that this cancer is generally diagnosed in advanced stages and is one of the most deadly cancers. This disease has a very complex pathogenesis, and researches so far have not been able to determine the main reason and how this disease progresses, this shows the importance of investigating the expression profile of genes in this disease to reveal the molecular process of the pathogenesis of this disease. In the same direction and in order to improve the knowledge of the molecular pathways causing pancreatic cancer, this study was conducted with the aim of determining the expression profile of LINC01111, miR-3924 and the expression level of DUSP1 in pancreatic adenocarcinoma tumor and margin samples. Methods: Tissue samples, including 50 pancreatic cancer tissue samples and 50 margin tissue samples, were prepared and subjected to RNA extraction. Complementary DNA was synthesized and then expression levels of miR-181a and Caprin-1 genes were measured using q-PCR. In addition, the relationship between the expression of the studied genes with each other and the clinical pathological characteristics of the patients was evaluated. Results: The results obtained from q-PCR showed that the two investigated targets, LINC01111 and DUSP1, had significant expression changes in tumor samples compared to the margin. Meanwhile, the expression level of miR-3924 in Tumor samples had an increase compared to healthy tissue, but this increase was not statistically significant (p<0.05). In addition, the expression of DUSP1 had a negative correlation with miR-3924, but it was not related to the expression of other studied genes.