Analysis of correlation between glucocorticoid receptor polymorphisms and avascular necrosis in renal allograft recipients

Abstract

Glucocorticoids are known for their anti-inflammatory and immune inhibitory properties by acting on lymphocytes and inflammatory cells by inhibiting the κβNF pathway. By acting on their target cells, GCs act primarily by inhibiting the transcription of proinflammatory cytokines and inhibiting the production and proliferation of helper, cytotoxic, and inhibitory T cells (via apoptosis). In fact, corticosteroids, including glucocorticoids, play a key role in the development of AVN. Many of the effects of GC are caused by glucocorticoid receptors, so it can be said that GR is responsible for different responses to GCs. Therefore, we hypothesized that the presence of NR3C1 gene polymorphisms may be associated with the development of AVN in kidney transplant patients. Method: Blood samples were taken from 91 kidney transplant patients and the presence of AVN in symptomatic patients was determined by appropriate diagnostic methods. DNA was then isolated from whole blood and some DNA was loaded on agarose gel and spectrophotometry was used. Then, using designed primers, PCR steps were performed in a thermal cycler. Results: In this study, no association was found between the polymorphism of glucocorticoid receptor genes and the incidence of AVN in kidney transplant patients. In other words, a total of 15 kidney transplant patients studied in this study had at least one of the glucocorticoid receptor polymorphisms in the study, none of which had AVN.