Examining the expression of genes identified through the analysis of gene expression networks through system biology in colorectal cancer cells

Abstract

Colorectal cancer is one of the most common cancers of the gastrointestinal system, which is the third most common cancer and the second deadliest cancer. Unfortunately, due to the late diagnosis and the high use of chemotherapy drugs during the treatment of this type of cancer, most of the colorectal cancer patients with drug resistance to drugs, especially 5-FU drug, show that this resistance can develop before the treatment with this drug and or after consuming it. Studies have shown that expression changes at the level of key genes can accelerate or affect the induction of drug resistance in patients with colorectal cancer. Methods: In this study, using the data available on the Gene Expression Omnibus (GEO) website about gene expression in 5-FU-resistant colorectal cancer cell samples, genes with altered expression were identified and these genes have been evaluated to investigate the expression change. Results: After resist the colorectal cancer cells in the laboratory and examining the expression of candidate genes, the results showed that MMP7, CLDN1, ADH1B, CDH3, ABI3BP genes have altered expression.