Effect of intravenous injection of bone marrow-derived C-kit⁺ cells on the signaling pathway of IL33/ST2/ILC2, IL2, IL10 in the lung tissue of type 2 diabetic male rats

Abstract

Inflammation is an essential factor in spreading diabetes and its pulmonary complications. Bone marrow (BM)-derived C-kit⁺ cells have immunomodulatory properties. This study aimed to evaluate the effect of BM-derived C-kit⁺ cells on the inflammation signaling pathway in lung tissue of type 2 diabetes male rats. Methods: 50 male Wistar rats weighing 180±20 gr were used. 10 rats were randomly selected to extract C-kit cells, and the rest of the rats were randomly divided into four equal groups, including Control (Cont; 50 μl-normal saline solution-intravenously), Diabetes (D; induction diabetes by receiving high-fat diet+35mg/kg streptozotocine-intraperitoneal), Diabetes+C-kit⁺ (D+C-kit+, 50 μl-normal saline solution containing 300,000 C-kit⁺ cells-intravenously), and Diabetes+C-kit- (D+C-kit-; received 50 μl-normal saline solution containing 300,000 C-kit- cells-intravenously). After completing the interventions and induction of deep anesthesia, the right lung was taken out of the animal's body and used to determine the level of expression of Interleukin (IL)-33, ST-2, CD127, IL-2, and IL-10 by Western blotting. The left lungs are used for histopathological study(H&E). Results: Induction of diabetes significantly increased the levels of IL-33, CD127 and IL-2 and decreased IL-10. Injection of C-kit⁺ cells significantly decreased the level of IL-33 and CD127 and increased IL-10 in mice (p<0.05). Although the injection of C-kit- cells decreased IL-33, CD127, and IL-2, this decrease was not significant. However, it significantly decreased the level of IL10 compared to the C-kit⁺ group (p< 0.05). Histopathological changes in the lung tissue in the group receiving C-kit⁺ cells showed a significant improvement compared to the diabetes group.