Evaluation of the effect of human adipose tissue-derived mesenchymal stem cells (AD-MSC) on gene expression, methylation and levels of inflammatory cytokines in PBMCs of patients with chronic obstructive pulmonary disease (COPD)

Abstract

Inflammatory responses play a significant role in the development and progression of COPD. Recent studies have shown. So far, various treatment methods have been proposed to treat these patients. In this study, we investigated the effect of mesenchymal stem cells on gene expression, promoter methylation and inflammatory cytokine secretion by peripheral blood mononuclear cells of patients with COPD as a therapeutic method. Methods and Materials: In this experimental study, 8 cc of peripheral blood was taken from 20 patients with COPD in order to isolate PBMC. After sampling and isolating mononuclear cells (PBMC) from them, the level of gene expression, methylation and the level of secretion of cytokines IL-6-IL-18, IFN-γ and TNF-α respectively using the method RT-PCR and ELISA were measured before and after co-culture with human MSCs. Finally, Graphpad Prism software was used for data analysis, and p<0.05 is considered as a significant level. Findings: Based on our results, after co-cultivation, the gene expression and secretory level of inflammatory cytokines in peripheral blood PBMC of patients decreased significantly. The methylation level of all inflammatory cytokine genes also increased after co-cultivation, which is significant in a number of genes.