The role of miR-205-5p in proliferation and susceptibility of osteosarcoma cancer cells in presence of Doxorubicin drug

Abstract

Recently, various studies have focused on the therapeutic potential of miRNAs, especially miR-205-5p, which is one of the tumor suppressor miRNAs, in various human malignancies, including osteosarcoma. However, the underlying mechanisms in miR-205-5p-mediated anticancer effects are still not fully understood. Therefore, the present study investigated the effect of miR-205-5p on doxorubicin (DOX)-induced apoptosis in Saos-2 cells and resistant cells. Methods: Saos-2 and Saos-2/DOX cells were treated with DOX, miR-205-5p and a combination of both, and cell viability was evaluated by MTT method. The expression of miR-205-5p, PTEN and MDR1 was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Flow cytometry method was also used to investigate apoptosis. Results: As expected, miR-205 was down-expressed in Saos-2/DOX cells (P<0.05). Overexpression of miRNA-205 was shown to significantly inhibit the viability of Saos-2/DOX cells. Increased expression of miR-205 significantly decreased the expression and activity of MDR1 in DOX-resistant cells (P<0.05). PTEN is an important modulator of the PI3K/Akt/MDR1 cascade, and expression of miR-205 led to increased PTEN and sequentially lower MDR1 expression, which reversed drug resistance. Also, overexpression of miR-205 increased the apoptosis rate of Saos-2/DOX cells