Evaluation of the role of miR-205-5p in proliferation and susceptibility of prostate cancer cells in the presence of 5-fluorouracil

Abstract

Recently, various studies have focused on the therapeutic potential of miRNAs, especially miR-205-5p, which is one of the tumor suppressor miRNAs, in various human malignancies, including prostate cancer. However, the underlying mechanisms in miR-205-5p-mediated anticancer effects are still not fully understood. Therefore, the present study investigated the effect of miR-205-5p on metastasis and apoptosis induced by 5-fluorouracil in LNCaP cells. Methods: LNCaP cells were treated with 5-fluorouracil, miR-205-5p and a combination of both, and cell viability was assessed by MTT assay. miR-205-5p expression was assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Important markers of metastasis including MMP-2, MMP-9, c-Myc, CXCR4 and K-Ras and apoptosis markers Bax and Bcl-2 were evaluated using Western blot. Flow cytometry method was also used to investigate apoptosis. Results: 5-fluorouracil resulted in significant inhibition of cell proliferation in a dose-dependent manner. The combination of miR-205-5p and 5-fluorouracil led to a significant inhibition of cell proliferation compared to single treatments (P<0.05). miR-205-5p also significantly inhibited the metastasis of LNCaP cells by inhibiting the expression of metastasis markers including MMP-2, MMP-9, c-Myc, CXCR4 and K-Ras. Furthermore, miR-205-5p increased 5-fluorouracil-induced apoptosis in LNCaP cells.