The use of silver nanoparticles to develop an analytical method for quantification of trientine

Abstract

Silver nanoparticles have a positive surface charge that could be changed the surface plasmon by adding materials with different properties. They have UV absorption which could be reduced in presence of different concentrations of analyte. Modified silver nanoparticles by anionic surfactants can be used for the measurement of amines. Trientine that is used in Wilson’s disease, is aliphatic amine which does not have UV absorbing groups. Aims: In this study, the modified silver nanoparticles by sodium lauryl sulfate have been used to develop an analytical method for quantification of trientine. Method: Silver nanoparticles were produced using a solution of silver nitrate, sodium lauryl sulfate (SLS) and sodium borohydride as the reducing agent. Different concentrations of trientine were added into a certain concentration of silver nanoparticle and absorbance of each sample was measured at 397 nm under the optimal conditions which include pH, salt and nanoparticle concentrations and time. It was optimized by a design of experiments using response surface methodology. Then, the calibration curve was obtained.The accuracy and precision of the developed method were examined. Selectivity of the developed method was performed in plasma and in presence of common cations.Results: Under optimum conditions (pH=2, NaCl concentration= 5×10-4 M, AgNPs volume= 1.5 ml, and time= 15 minutes), linear range of this method was between 10-40 ng/mL with correlation coefficient (R2) of 0.996 with limit of detection and quantification of 3 ng/mL and 10 ng/mL, respectively. The method had appropriate selectivity after dilution up to the concentration of drug in plasma and in presence of common cations.Discussion and conclusion: In this study, the modified silver nanoparticles were developed to measure trientine in aqueous and plasma samples. This method is suitable and low costing and does not require high equipment.