Anti-aging effect of barley stem cell extraction loaded nanoliposome on facial skin

Abstract

Introduction: Agents such as sun radiation may cause skin aging and lyperpigmentation. Using plants in cosmetic treatments is popular as they have fewer side effects. Utilizing plant stem cells is a new approach to formulating cosmetic creams that are plant-based. Plant stem cells are rich in antioxidants, growth factors, and many other compounds, therefore they are an invaluable resource of plant-based active ingredients. Barley has had various applications since a long time ago because of its many different properties recent studies have shown the anti-melanogenesis and whitening effects of the barley plant.Goal: We aimed to Investigating the anti-aging effects of extract from barley plant stem cell culture loaded in nanoliposome on human skin.Methods: Nano-liposomes were prepared by a thin layer method and characterized for size, Polydispersity Index (PDI), zeta potential (ZP),SEM, entrapment efficiency (%EE) and loading capacity (%LC). The in-vivo experiments on 12 volunteers were conducted with the barley extraction on nano-liposome on the right side of the face around forehead and the solution form of extraction on the other side of the face symmetrically, and the effect of these two formulations on reducing wrinkles around the forehead were compared.Results: The developed Nano-liposomes showed proper characteristics for dermal delivery of barley callus extract such as mean particle sizes around 120nm and EE and LC values of %86.9 and %17.56 respectively. In-vivo studies in humans showed the superiority against the controls in the collagen generation in the skin and skin surface smoothness.Conclusion: It was concluded that the nanoliposome formulation containing barley callus extract can be introduced for local administration of barley callus extract in the prevention or treatment of photoaging.