The effect of voluntary exercise on the path of activity Keap1 / Nrf2 and the levels of NF-KBand IL-6 proteins in the heart tissue of male rats on a high-fat diet

Abstract

Today, changes in lifestyle and diet cause a series of diseases in the society. Consuming a high-fat diet can contribute to the development and progression of diabetes, as well as the risk of obesity, in addition to cardiovascular diseases. Since obesity is related to the process of inflammation and heart diseases, but the possible mechanisms of this relationship are not fully known yet, in this study, we examined the effect of high-fat diet on the process of inflammation through the Nrf2/Keap1 signaling pathway, as well as the effect of voluntary exercise. Materials & Methods: In this study, 28 male Wistar rats were randomly divided into 4 groups (n = 7). 1) Control group: without any intervention. 2) High-fat diet group: In this group, the animals received a high-fat diet for 10 weeks. 3) Voluntary exercise group: In this group, the animals did voluntary exercise for 10 weeks. 4) High-fat diet group and voluntary exercise: In this group, the animals received a high-fat diet and exercised voluntarily for 10 weeks at the same time. Voluntary exercise was performed for 10 weeks by placing the animal in a special cage with a rotating wheel. At the end of the intervention, the animals were anesthetized and The animal's sternum and heart will be removed to check the amount of NF-KBand IL-6 proteins in the Nrf2/Keap1 pathways and will be frozen in liquid nitrogen and kept at minus 70 degrees Celsius until the western blot measurement.

Results: Compared to the control group, while the high-fat diet caused a significant increase in IL-6 and Keap1 levels (p<0.05). Exercise alone did not affect these parameters and only combined with high-fat diet caused a significant decrease in them (p<0.05). Compared to the control group, the level of Nrf2 expression decreased in the high-fat diet group and increased significantly in the voluntary exercise group (p<0.05). However, no significant change was observed in the group with voluntary exercise and high-fat diet.