The stereological investigation of the effect of neurotrophic factor- secreting cells on volume changes in different parts of the hippocampus in a model of Alzheimer's disease

Abstract

Alzheimer's disease is the fifth cause of death around the world. The hippocampus which is the first regions involved via AD and atrophy. Neurotrophic factors secreting cells play role in improving the symptoms of Alzheimer's disease. As a result, current study investigated the stereological investigation of the effect of neurotrophic factor- secreting cells on volume changes in different parts of the hippocampus in a model of Alzheimer's disease. Methods: In the present study, 30 mice were divided into control group, AD + DMEM group, and AD + supernatant group. 21 days after intraventricular injection of amyloid beta 1-42 protein, AD model was confirmed using the shuttle box device. Then the supernatant of NTF-SCs cells and DMEM were injected into the groups for treatment, and after 28 days, the brains of the mice were collected and studied with chrysl violet staining for stereological examination. Results: our results showed that amyloid beta injection in the Alzheimer's disease mice model decreased the step through latency time in the AD + PBS group. The results of chrysl violet staining of the DG area of the hippocampal reduced the number of neurons following amyloid beta injection, while the NTF-SCs supernatant recovered and regenerated the number of the neurons in DG. Total volume of the hippocampus in the control and AD + PBS groups shows a decrease in the total volume and sub-regions of the hippocampus after the injection of amyloid beta (P<0.001). While the comparison of the AD + NTF-SCs group with the AD + PBS group shows the improvement of the total volume and subregions of the hippocampus.