Investigation of the lncRNA LINC01133 expression in the tumoral and margin tissue of the malignant thyroid tumor

Abstract

Thyroid cancer is one of the most common endocrine cancers worldwide, especially among women. The role of non-coding RNAs, including lncRNAs, in the development of various diseases, especially cancer, has been reported. Considering the prominent role of LINC01133 in other human malignancies, the purpose of this study is to investigate the level of LINC01133 gene expression in cancerous and healthy thyroid tissues with diagnostic and therapeutic purposes. Methods: At first, 25 thyroid cancer tissues and 25 healthy adjacent tissue samples were taken from thyroid cancer patients with written consent. Also, their clinical and pathological characteristics were determined by the pathologist. RNA related to the samples was extracted and after checking and confirming their quality and concentration, cDNA was synthesized. Next, the expression of LINC01133 lncRNA in the samples was measured by real-time PCR method and its relationship with clinical and pathological characteristics was investigated. Also, the expression of LINC01133 was bioinformatically evaluated using TCGA database data in thyroid cancer samples compared to the samples obtained. Finally, statistical calculations were performed to determine the biomarker power of this lncRNA. Findings: The results of the study showed that LINC01133 gene expression significantly decreases in thyroid cancer samples in laboratory samples and TCGA samples. ROC curve analysis indicated the high value of the biomarker expression profile of LINC01133 in thyroid cancer samples. Further informatics analysis showed that LINC01133 can play a role in processes such as extracellular matrix organization.