Evaluation of expression of the relationship between the expression of UCA1 lncRNA and miR-143 genes in tumor tissue and tumor margin tissue in patients with melanoma

Abstract

Melanoma is caused by the out-of-control proliferation of skin melanocyte cells. The high growth and metastasis of cells in this cancer has made this cancer one of the deadliest cancers. This disease has a very complex pathogenesis, and researchers so far have not been able to determine the main reason and how this disease progresses, this shows the importance of investigating the expression profile of genes in this disease to reveal the molecular process of the pathogenesis of this disease. In this regard and to improve the understanding of the molecular pathways that cause melanoma, this study was conducted with the aim of determining the expression profile of UCA1 lncRNA and the expression level of miR-143 in tumor samples and melanoma margins. Methods: Tissue samples, including 50 melanoma cancer tissue samples and 50 margin tissue samples, were prepared, and subjected to RNA extraction. Complementary DNA was synthesized and then the expression levels of UCA1 lncRNA and miR-143 genes were measured using q-PCR. In addition, the relationship between the expression of the studied genes with each other and the clinical pathological characteristics of the patients was evaluated. Results: The results obtained from q-PCR showed that both studied genes had significant expression changes in tumor samples compared to the margin (p>0.05). It was increased compared to healthy tissue, but these changes in the expression of miR-143 in a tumor sample were a decrease compared to healthy samples. In addition, the expression of the two desired genes in tumor samples had a negative correlation.