The effect of BV6 and doxorubicin dual delivery by SPION nanoparticles on cancer cell survival

Abstract

One of the prominent characteristics of cancer cells is resistance to apoptosis. After initial sensitivity to chemotherapy, many types of cancer cells become resistant to apoptosis by changing the expression of factors involved in cell survival. Such behavior has also been observed abundantly in the behavior of all types of cancer cells towards doxorubicin. Therefore, finding ways to increase the sensitivity of cancer cells to doxorubicin is one of the most important research fields. Apoptosis inhibitors are increased in many cancer cells. Therefore, inhibition of apoptosis inhibitors can be an effective way to increase the sensitivity of cancer cells to doxorubicin. BV6 drug is one of the most important suppressors of apoptosis inhibitors, which is highly effective in inducing cell death. Materials and methods: In this study, biodegradable nanoparticles were used to deliver the drugs doxorubicin and BV6 to cancer cells. This study was used on mouse cancer cell lines including 4T1 and CT26. The effect of combined treatment on cell death was evaluated by MTT and apoptosis methods, and the effect of treatment on the expression of genes involved in apoptosis was evaluated by Real-time PCR method. Result: The result of this study showed that the combined treatment of cancer cells with nanoparticles loaded with doxorubicin and BV6 induces death in cancer cells. Also, the treatment of cells decreased the expression of anti-apoptotic factors and increased the expression of pro-apoptotic factors.