TGF-B effect on M-CSF and M-CSF in Melanoma cell line A-375, C6181

Abstract

Cancer includes a group of diseases whose main characteristic is unregulated cell growth, invasion and spread of cells from the primary site to other parts of the body. Despite recent advances in diagnosis and treatment, cancer is one of the most important causes of death in the world. Skin cancer is one of the most common cancers in the world. One out of every three cancers diagnosed in the world is skin cancer, and the importance of early treatment is increasing day by day. Melanoma is the most aggressive and deadly type of skin cancer and one of the most malignant cancers. Various methods such as radiotherapy, chemotherapy, immunotherapy and surgery are used in the treatment of this type of cancer. The purpose of this study is to investigate the effect of TGF-B on human G-CSF and M-CSF gene expression. Materials and methods: The present laboratory study was performed on melanoma cancer cells in RPMI640 culture medium containing 10% FCS at 37ₒc and 5% CO2. After counting and dividing the cells, the inside of the cell plate was stimulated with TGF-B protein as a time course. Incubation was done. After 48 hours, the cells were collected and total mRNA was extracted and the expression level of M-CSF, G-CSF with TGF-B stimulation was checked through qRT-PCR. SPSS software was analyzed and P<0.05 was considered significant in all statistical tests. Results: The results of the study showed that the expression level of G-CSF, M-CSF decreases with TGF-B stimulation in both cell lines.