Effect of Intradermal lymphocyte therapy on expression levels of miRNA-223, miRNA-133b in Peripheral Blood Mononuclear Cells of patients with recurrent pregnancy loss

Abstract

In recent studies, the discussion and exchange of opinions in the field of immunotherapy for reproductive patients, especially those with recurrent miscarriages (RPL), is very interesting. Limited studies are dedicated to investigating the effect of intradermal lymphocyte therapy in improving the pregnancy rate in women with RM. Therefore, we decided to investigate the effect of intradermal lymphocyte therapy on pregnancy success in patients with recurrent miscarriages, its effects and the function of a group of some miRNAs including microRNA-223 and microRNA-133b in these people. Methods: 25 patients with recurrent miscarriage and 25 healthy people with successful pregnancy were considered as the control group for this study. To prepare peripheral blood mononuclear cells (PBMCs) from peripheral blood, first 10 ml of heparinized blood is taken from patients and peripheral blood mononuclear cells are isolated using Ficol 1.077. According to the standard protocol, the number of 2 × 107 was injected subcutaneously to patients with recurrent miscarriage. The number of injections is three times with one-month intervals. Real-time PCR technique was used to check the expression level of microRNA-223 and microRNA-133b molecules. Findings: In this study, the gene expression level of micro-RNA 223 in RPL women before three cycles of subcutaneous injection of lymphocytes was 2.122±1.131 and the gene expression level of the same micro-RNA after the lymphocyte therapy of the mother was 1.220±0.4391. Also, the expression level of micro-RNA 133b gene in these women was reported to be 1.678±0.7449 before treatment and 1.177±0.4480 after three cycles of treatment with paternal lymphocytes. This level of gene expression in women with a history of successful pregnancy as a control group, for micro-RNA 223 and micro-RNA 133b genes is 1.000±0.07882 and 1.000±0.09502, respectively.