Investigation of the toxicity, stability and antimicrobial effects of liposomes containing oleuropein.

Abstract

Introduction: Oleuropein is a phenolic compound whose many applications as an antimicrobial agent in food are associated with problems such as degradation due to environmental conditions and unpleasant sensory characteristics, including bitterness. The purpose of this thesis is to achieve a form of this effective substance that has better efficiency and less undesirable characteristics (1).Objective: To investigate the toxicity, stability and antimicrobial effects of oleuropein liposomes against Staphylococcus aureus and Candida albicans.Methods: Nanoliposome containing oleuropein was prepared based on the freeze-drying method of a monophase solution.Then, the release in hydrophilic environment, stability, XRD, FTIR of liposomes containing oleuropein and toxicity level were studied by MTT assay on human fibroblast cells, as well as the antimicrobial properties of liposomes containing oleuropein against Staphylococcus aureus and Candida albicans using microdilution method.Results: Investigation of antimicrobial properties showed that nanoliposome containing oleuropein improves the antimicrobial effect of oleuropein on 2 microorganisms S/ aureus and C/ albicans/ Based on the release results in the hydrophilic medium, oleuropein is slowly and continuously released from nanoliposomes(7% in 72 hours). In the FTIR study, functional groups observed for oleuropein are also seen for nanoliposomes containing oleuropein, which indicates that the substance is loaded inside the liposome. XRD diagrams show the amorphousness of liposomes containing oleuropein. The stability test results show that the stability over time is better at 4 degrees Celsius. In the cytotoxicity test, increasing the concentration of liposomes containing oleuropein showed a decrease in the survival rate of fibroblast cells.Conclusion: It is possible to prepare nanoliposomes containing oleuropein with suitable properties and increased antimicrobial effects by Using the modified lyophilization of mono-phase solution method.