Correlation between CMV and BK infections with Aquaporin 5(−1364A/C) Promoter Polymorphism in renal transplant patients admitted to Tabriz Imam Reza Hospital

Abstract

CMV and BK virus infection occurs chronically in kidney transplant patients. AQP5 polymorphism may be involved in the risk of CMV and BK in kidney transplant recipients due to their resistance to viral infections, but the data on this subject do not fully support this. Accordingly, we will test the hypothesis that AQP5 polymorphism is associated with the risk of CMV and BK in kidney transplant recipients. Materials and methods: In this study, 150 research units that underwent kidney transplantation in Imam Reza Hospital in Tabriz in 2011-2012 were included in the study. 50 transplant recipients with viral infection (CMV and BK), 50 transplant recipients without viral infection and 50 healthy individuals were compared. Blood samples were taken from three groups of patients for DNA extraction and the studied polymorphism of aquaporin 5 (1364A / C-) was examined by ARMS PCR. Then, all the patients' information obtained from the interview was obtained and the information obtained from the laboratory was entered into the checklists. Finally, all the information was entered into SPSS v21 statistical analysis program and we analyzed the data. Results:In this study, 150 research units were examined. 34 patients (68%) were in CMV + group, 26 patients (52%) were in CMV- group and 31 patients (62%) were in male control group (P = 0.254). The mean age in the CMV + group was 45.32 13 13.12 years, in the CMV- group was 40.82 9 9.9 years and in the control group was 44.72 9 9.44 years (P = 0.086). In the CMV + group, 38 patients (72%) and in the control group, 43 patients (86%) received a kidney transplant from a live donor (P = 0.202). 13 patients (26%) in CMV + group and 8 patients (16%) in CMV + group had a positive history of acute kidney transplant rejection (P = 0.220). In this study, CC genotype was seen in CMV + group in 4 patients (8%), in CMV- group in 2 patients (4%) and in the control group no disease with this genotype was seen (P = 0.354). Data analysis showed that there was no association between AQP5 genotype with CMV infection or healthy unrelated individuals.