Effects of different concentrations of aluminum phosphide and sodium bromide, calcium carbonate and Potassium nitrate as its probable antidotes on apoptosis related genes expression in lung and liver of rats

Abstract

Rice tablets are among the most common causes of suicide and acute poisoning in our country and third world countries, and so far its mechanism of action has not been well defined and its antidote has not been identified. The aim of this project is to investigate the effect of 3 substances as proposed antidotes to reverse apoptosis induced by aluminum phosphide tablet poisoning in rats. Methods: One hundred and two male Wistar rats were divided into different groups to investigate different concentrations of AlP as well as the compounds of sodium bromide, calcium carbonate and potassium nitrate. According to the results of the first part of the study and the quick death of 4 out of 6 rats receiving a dose of 40 mg, a dose of 20 mg/kg of rat weight was selected for the main study. Then low and high doses of each antidote were administered to the rats. Liver and lung tissues were removed and the expression levels of TNF, IL-6, p53, Bax, Bcl-2, Caspase-3, Caspase-8 and Caspase-9 genes were measured using qRT-PCR technique. Findings: There was a significant difference in the expression of TNF gene in the lungs of the high-dose potassium nitrate group compared to the ALP group. The group receiving ALP along with calcium carbonate had a significant difference in p-53 gene expression in the lung with the group receiving ALP. The highest expression of Caspase-9 gene in the liver was in the group receiving ALP along with potassium nitrate, which was significantly different from other groups. Regarding Caspase-8 gene expression, the highest gene expression was in the group receiving ALP along with potassium nitrate at a low dose, which had a significant difference compared to other study groups. The highest expression of Caspase-3 gene in liver and lung was in the group receiving ALP and low dose potassium nitrate, which was significantly different from other groups. The highest expression of IL-6 gene in the liver was in the group receiving ALP along with low dose calcium carbonate, which was significantly different from other study groups. In liver and lung, BAX gene expression was highest in the group receiving ALP along with potassium nitrate, which was significantly different from other studied groups.