Evaluation of the effect of TNF alpha on the expression of G-CSF, M-CSF in monocyte cells of MS patients

Abstract

MS is an inflammatory autoimmune disease in which the myelin sheaths of nerve cells in the brain and spinal cord are damaged. This damage can disrupt the ability of parts of the nervous system that are responsible for communication and cause many physical signs and symptoms. G-CSF is a major extracellular regulator of hematopoiesis and the innate immune system. M-CSF is a glycoprotein that is effective in hematopoiesis and stimulating the production of numerous cytokines. It also plays a key role in stimulating the clearance of myelin by the brain's innate immune cells, which is a prerequisite for proper remyelination and myelin repair. The aim of the study is to investigate the effect of overlapping and non-overlapping cytokines in MS.

Materials and methods: 20 MS patients referred to the MS clinic of Imam Reza Hospital with mild, moderate and severe stages were selected, and blood samples were taken from the patients and MACS monocyte cells were separated from PBMC. Monocyte cells were evaluated by flow cytometry with CD14 antibody to evaluate the purity percentage. The monocyte cells were then cultured and stimulated with TNF α as a time course of 0, 6, 12, 24 and 48 hours. After the end of stimulation, the cells were collected and the expression level of G-CSF, M-CSF was evaluated by Real Time PCR. Findings: G-CSF and M-CSF receptors expression decreased significantly with the passage of time as a time course of stimulation, and this decrease reached its lowest value 12 hours after stimulation. Comparing G-CSF and M-CSF, the decrease ratio has been the same.The level of TNF α function was evaluated as a time course.