Investigating the effect of red blood cell lysate on the fatty acid pattern of serum phospholipids

Abstract

Serum phospholipids play an important role as polar molecules by forming lipoproteins. On the other hand, any change in the pattern of serum fatty acids can also cause changes in cell signaling pathways. In intravascular hemolysis, the destruction of red blood cells is accompanied by the release of intracellular contents into the blood plasma. Therefore, the purpose of this study is to investigate the effect of red blood cell lysate on the fatty acid pattern of serum phospholipids (in vitro). Methods: This study was experimental research on human samples. In this study, serum samples and red blood cells were obtained from the 5 (20-50 y) healthy adults’ men. Serum was separated from 5 ml of blood without anticoagulant and red blood cells were separated from 5 ml of blood using EDTA anticoagulant. Separation of phospholipid fraction was done using thin layer chromatography method and methyl ester of fatty acids will be done in acidic environment by methanol.Results: The amount of trans-palmitoleate fatty acids (1:16) and oleate (1:18) at 37 oC showed a significant decrease compared to the control group (respectively -20.74%, p=0.001 and 10.08% ± 0.2, p=0.01). In contrast, cis-palmitoleate fatty acid (16:1c) at 37 oC temperature showed a significant increase compared to the control group (44.15% ± 0.11, p=0.001). The amount of fatty acids linoleate (18:2), linolenate (18:3), arachidonate (20:4) and docosahexaenoic acid (DHA) at 37 oC temperature showed a significant decrease compared to the control group. The amount of total saturated fatty acids (SFA) at 37 oC temperature showed a significant increase compared to the control group (9.11% ± 0.82, p=0.01). On the other hand, the total amount of monounsaturated and polyunsaturated fatty acids at 37 oC temperature showed a significant decrease compared to the control group (-8.69% ± 2.15, p=0.01 and 94% ± 0.52, respectively)