Investigating the effect of decreasing SLC16A13 gene expression on apoptosis and metastasis in Larynx cancer cell line

Abstract

Tumors in the oral cavity are either benign or malignant like the rest of the body. Eighty-five to ninety-five percent of all cancers in the oral cavity are squamous cell or squamous cell carcinoma (SCC), which is very dangerous and deadly. Genetics and environment have a separate and clear role in this cancer, although the role of environment in this disease is more pronounced than genetics, but these environmental changes ultimately lead the cell to cell death by creating genetic changes. SLC16A is a member of the family of membrane transporters whose importance in the process of carcinogenesis has recently been emphasized. In the present study, we tried to knock down the expression of SLC16A gene in laryngeal cancer cell line (Detroit 562) to investigate the effect of reducing the expression of this gene on the ability of survival, growth and metastasis in these cells. Matherial and methods: At first, Larynx cancer cell lines (Detroit 562) were cultured in standard medium and then transfected into the target cells to reduce the expression of the target gene, synthetic siRNA of SLC16A13 antigen. Then, we used MTT and flow cytometry tests to investigate the effect of reducing the expression of the studied gene on the process of cell death and apoptosis. SCRATCH test was also used to investigate the role of this treatment on the migration ability of cancer cells. Also, the effect of this treatment on the change of target gene expression will be analyzed by Real Time PCR. Finally, the expression changes of genes involved in apoptosis and cell migration were evaluated by Real Time PCR method. Results: According to the results obtained from the present study, the reduction of SLC16A13 gene expression causes an increase in the apoptosis rate in lung cancer cells, but does not change the ability of cancer cells to migrate.