study The effect of human AD-MSCs derived exosome on gene expression, methylation and levels of inflammatory cytokines in PBMCs of patients with chronic obstructive pulmonary disease (COPD)

Abstract

Chronic obstructive pulmonary disease is the most common cause of death and disability caused by lung diseases. Chronic obstructive pulmonary disease is associated with chronic obstruction of airflow into or out of the lungs. Today, research on extracellular vesicles derived from mesenchymal stem cells is one of the areas of interest in regenerative medicine and inflammatory diseases. The present study, for the first time, seeks to investigate the regulatory effects of exosomes derived from MSC cells on the level of gene expression, methylation and secretion of inflammatory cytokines in PBMCs of COPD patients. Methods and Materials: In this experimental study, 8 cc of peripheral blood was taken from 20 patients with COPD in order to isolate PBMC. After sampling and isolating mononuclear cells from them, the level of gene expression, methylation and the secretory level of cytokines IL-6, IL-18, IFN-γ and TNF-α respectively using RT-PCR method and ELISA was measured before and after co-culture with human MSCs. Finally, Graphpad Prism software was used for data analysis, and p<0.05 is considered as a significant level. Results: Based on our results, after co-cultivation, the gene expression and secretory level of inflammatory cytokines in peripheral blood PBMC of patients decreased significantly. The methylation level of all inflammatory cytokine genes also increased after co-cultivation, which is significant in a number of genes.