Isolation and recognizing of the clinical isolates of Cryptococcus complex isolated from patients admitted to Sina Hospital in Tabriz

Abstract

Background. Over the past few decades, several factors such as the increasing in the usage of antibiotics and immunosuppressive drugs, as well as the increasing in the number of people infected with the immunodeficiency virus (HIV) and long-term hospitalizations, especially in the ICU department, have caused the increasing in the number of immunocompromised people. Aim: The aim of this study was to isolate and identify clinical isolates of Cryptococcus neoformans complex and Cryptococcus getti from patients admitted to Sina Hospital in Tabriz.method. The aim of this study was to isolate and identify clinical isolates of Cryptococcus neoformance complex and Cryptococcus gattii from patients admitted to Sina Hospital in Tabriz.findings. Samples were selected from hospitalized patients who are suspected of invasive fungal infection and especially have cerebral (meningitis and meningoencephalitis) and pulmonary symptoms. The number and type of samples taken from each patient depends on the symptoms in the patient, which include cerebrospinal fluid, saliva, sputum, urine, feces, wound secretions, etc. The details of each patient were recorded in the information form. From the collected samples, after preparation (centrifugation, homogenization, etc.), two smears were prepared with Chinese compound dye and potassium hydroxide and examined under a microscope with 10 and 40 lens with the aim of seeing encapsulated yeast. It is cultured on Niger Agar medium. The plates were incubated for 3 to 5 days at 30°C.Conclusion. Classical laboratory methods are useful for identifying Cryptococcus neoformance after isolation from clinical samples of hospitalized patients and can be used in the diagnosis of these strains. In the current study, the positive culture result of one of the 234 samples examined in all three culture environments indicated the identification of Cryptococcus neoformance.