Evaluation of the effect of human mesenchymal stem cell exosomes on Th1 cells in patients with chronic obstructive pulmonary disease (COPD)

Abstract

Inflammatory responses play a significant role in the development and progression of COPD. Recent studies have shown that adult mesenchymal stem cells, such as bone marrow cells, help regenerate and protect lung tissue, and therefore the use of stem cells may be a powerful treatment to prevent inflammation caused by COPD and regenerate lung tissue. Methods and Materials: In this fundamental study, in order to isolate PBMC cells, 8 ml of blood containing anticoagulant was taken from 20 COPD patients. After sampling and separating PBMC cells from them, the differentiation percentage of TH1 cells and the level of gene expression and secretory level of IL-12 and IFNγ cytokines before and after co-cultivation; It was measured with exosomes derived from MSC cells. Finally, Graphpad Prism software was used for data analysis, and p>0.05 was considered as a significant level. Results: The results of the present study showed that the secretion of IL-12 and IFNγ cytokines significantly decreased after co-culture with MSC-derived exosomes. Also, the gene expression level of these cytokines and transcription factors STAT1 and STAT4 has decreased significantly. In addition, a significant decrease in the differentiation rate of lymphocytes to TH1 was observed after co-culture with MSC exosomes.