Evaluation of cardioprotective effects of taurine, crocin and grape seed extract on HCM cells due to aluminum phosphide poisoning

Abstract

Introduction: the protective effect of crocin, taurine and grape seed extract on the cardiotaxiticy of human cells in a cell culture medium is investigated. Goal: Aluminum phosphide is a well-known hazardous agent used as an agricultural pesticide to protect stored grains from insect damage. However, accidental consumption of a trivial amount of it caused irreversible damage to the human body or even death in acute cases. The present study used crocin, taurine and grape seed extract as a natural cardioprotective medicine against aluminum phosphide poisoning by decreasing oxidative stress. Method: The activity of oxidative stress biomarkers (Malondialdehyde, Catalase, Protein carbonyl, and Superoxide dismutase) were evaluated in the cell line model on Human Cardiac cells. Initially, to clarify the pure impact of aluminum phosphide poison, crocin, taurine, and grape seed extract on the human heart cells, their effects on the biomarkers quantity in cell line were measured. Subsequently, the effect of the mentioned agents with various concentrations on the oxidative stress biomarkers of the poisoned heart cells were studied. Results: Data analysis reveals that crocin, taurine and grape seed extract treatment can successfully diminish the poisoning effect by their antioxidant properties. The oxidative markers values of the poisoned cells were recovered by taurine and grape seed extracts treatment. Taurine (2 g/l) can recover Malondialdehyde, Catalase, Protein carbonyl, and Superoxide dismutase by 56%, 78%, 88%, 78%, when the recovering power of grape seed extract (100 g/l) for the aforementioned enzymes are 56%, 71%, 74%, 51%, and for crocin (7 g/l) around 56%, 76%, 70%, 58%, respectively. Disscussion: Performance of taurine in the recovery of the biomarkers’ value is better than crocin and grape seed extract, while all the agents has positive effect in reducing the cardiotoxiticity.