Comparison of of expression level of miR-497 miR-378 and miR-103 in tumoral and marginal tissues of Melanoma

Abstract

Melanoma is one of the most common cancers with rapid spread, especially in areas with intense light radiation, which has a high mortality due to high metastasis. Together with pancreatic cancer, these cancers are known to be the most deadly cancers that have an unknown pathogenesis. micro_RNAs are an important class of RNAs whose expression and epigenetic changes have been evaluated and confirmed in several cancers. In this study, the aim of this study was to investigate the relative changes in the expression levels of these genes in melanoma tumor samples. Methods: During this study, 50 tumor samples and 50 samples of marginal tissue around the tumor were collected as a control sample. After collecting RNA, the samples were extracted and the expression of the desired gene was evaluated using real-time PCR. Differences in expression levels of the two groups were assessed by appropriate statistical tests. In statistical tests, Pvalue <0.05 was considered significant. The results of this study showed that these genes can be used as diagnostic biomarkers of cancer. Results: The expression level of all three genes in tumor samples was significantly different from marginal samples. Also, the genes were significantly associated with patients' clinical features such as metastasis and lymph node involvement.