Evaluation of the frequency association of lncRNA H19 and lncRNA MALAT1 single nucleotide polymorphisms with recurrent miscarriage

Abstract

Recurrent miscarriage (RM) at a rate of 1% of all couples trying to conceive is one of the main problems in pregnancy. The root cause of the disease has not been determined, but genetic factors and lifestyle influence the process. In the genetic dimension, lncRNAs play a crucial role in their essential effects on molecular pathways. The minor change in the expression of lncRNAs shows its significant effects in various complications, one of which can be RM. SNPs are single nucleotide polymorphisms that have the potential to alter the function and expression of lncRNAs. Methods: This study investigated four SNPs in recurrent miscarriage with 400 cases and 400 controls in the Iranian Turkish Azeri population using ARMS-PCR, including the MALAT1 lncRNA rs619586, rs664589, and rs656605, and H19 lncRNA rs3741216 variants. Results: We found that the MALAT1 lncRNA rs619586G variant in the co-dominant (AA vs. AG; OR = 0.65, 95% CI = 0.47-0.9 and AA vs. GG; OR = 0.5, 95% CI = 0.26-0.99, P = 0.0074), the dominant (AG + GG vs. AA; OR = 0.63, 95% CI = 0.46-0.85, P = 0.0023) and over-dominant (AA+GG vs. AG; OR = 0.68, 95% CI = 0.5-0.93, P = 0.016) inherited models has an association with decreased risk of RM. Bioinformatics analysis also revealed the effect of variants on the ceRNA networks.