Evaluation of gene RASSF2 expression and methylation in tumor tissue and marginal tumor of colorectal cancer patients

Abstract

In this research, 75 tumor tissue samples and 75 tumor margin samples from patients with colorectal cancer were examined. After extracting RNA (trizol) from tissues, cDNA synthesis was performed and it was used to evaluate changes in RASSF2 gene expression by Real Time PCR. Also, DNA was extracted from tissue samples and the methylation pattern of RASSF2 promoter regions was investigated using bisulfite treatment. Statistical analysis was performed to investigate the changes in the expression and methylation of the RASSF2 gene and their effects on the clinical characteristics of the patients, and p. value<0.05 was considered as a significant level. Using the statistical method of ROC curve analysis, the biomarker potential of RASSF2 gene expression and methylation was evaluated as a factor in colorectal cancer diagnosis. Results: The obtained results have determined that there is a significant relationship between the gene expression level in cancerous tissue and healthy tissue, so that the expression of RASSF2 gene in colorectal cancer tissues is lower than in tumor peripheral tissues. Also, methylation in colorectal cancer tissues is clearly higher than healthy tissues.