Evaluation of Parathyroid Hormone Level with Expression of the Immune Checkpoint Inhibitory Factors in Hemodialysis Patients

Abstract

Patients with kidney function disorder have more sever immune disfunction compared to normal population. Studies have shown that both naïve and acquired immune system are disrupted. Result of this disruption on mortality and morbidity of these patients is significant. Immune checkpoints are part of normal immune system that paly a great role in cancer immunotherapy. In this study. We studied expression of these markers in patients undergoing hemodialysis with different parathyroid hormone levels. Method: 90 hemodialytic patients were entered to this study. Then they were divided into three groups consisting of 30 patients, based on their parathyroid hormone levels. Quantitative PCR and western blot techniques were used for analysis of expression of LAG-3, TIM-3, PD-1, CTLA4 genes and receptors. We used One Way Annova test for statistical analysis of the data and P-Value <0.05 was considered significant. Results: expression of LAG-3, TIM-3, PD-1 genes and receptors in patients with hyperparathyroidism was greater than in patients with hypothyroidism and patients with normal level of parathyroid hormone. However, CTLA-4 receptor and gene expression is lower in people with hyperparathyroidism than in people with hypoparathyroidism and people with normal levels of parathyroid hormone. TIM-3 receptor expression was significantly lower in patients with hypoparathyroidism than in those with normal parathyroid hormone levels.