Determination of blood levels of Voriconazole and Cyclosporine in allogen hematopoietic stem cell transplantation patients using green HPLC

Abstract

Introduction: Voriconazole is a broad-spectrum antifungal drug of the second generation of triazoles, which is the first line for the treatment and prevention of fungal infections, and cyclosporine is a drug with the property of weakening the body's immune system to prevent graft tissue rejection. Voriconazole and cyclosporine drugs have a narrow therapeutic window and their serum levels vary among people. Therefore, it is important to evaluate their serum levels. In most of the past researches, the effects on the environment have been ignored and solvents that are dangerous have been used. Today, efforts are being made to develop analytical methods that, while having high sensitivity and speed, help to maintain human health and environmental standards.Objective: To develop an analysis method based on green HPLC to evaluate the plasma concentration of voriconazole and cyclosporine in organ transplant recipients. Method: To precipitate plasma proteins, acetonitrile was added to the microtubes in a ratio of 1:1, and after centrifugation at 10,000 rpm for 10 minutes, the upper clear liquid phase was removed. 20 µl of it was injected into the device. Analysis of voriconazole was performed by HPLC-UV with isocratic flow of water: acetonitrile ratio 1:1 and analysis of cyclosporine was performed by LC-MS with gradient flow of acetonitrile: water: 0.3% formic acid.Results and findings: The results showed that the limit of detection and limit of quantification for voriconazole drug are 0.21 and 0.63 µg/ml, respectively, and for cyclosporine are 101.15 and 306.53 ng/ml, respectively was obtained. Conclusion: Based on the obtained data and results, the developed method for identifying and measuring voriconazole and cyclosporine in plasma can be used in clinical samples and pharmacokinetic studies.