Evaluation of the frequency of Treg cells and the expression of microRNAs and development factors of Treg cells in patients with premature ovarian failure

Abstract

Considering that POF is the main problem of women under 04 years of age and the negative consequences of this disease, which include infertility, increased risk of cardiovascular diseases, sexual dysfunction, and osteoporosis, therefore, we must look for effective factors in this disease and its consequences. It was from it. According to the studies that show that the role of the immune system in a successful and normal pregnancy and the birth of a live baby is very impressive, and the need for research and investigation in the field of its changes and disorders with the increase in the rate of infertility in today's world, more than it is obvious. Treg cells play an important role in preventing autoimmune diseases. Therefore, reducing the number or function of these cells can be effective in the occurrence of autoimmunity; As the defects of these cells have been reported in many autoimmune diseases, epigenetic changes are capable of changing the function of cells involved in fertility, and certainly their disorder or lack of regulation can be one of the factors involved in infertility.In this study, we investigate the frequency of Treg cells and the expression level of micro RNAs and factors affecting the development of Treg cells in patients with premature ovarian failure (POF).

Methods: In order to carry out this project, first, written and informed consent will be received from 30 women with premature ovarian failure (POF), who are our target population in this project. To start the study, heparinized blood is taken from these people and blood mononuclear cells (PBMC) are isolated. Then, flow cytometry technique is used to count the number of T reg cells. To check the expression level of micro RNA related to this cell, which is miRNA-155, we use the quantitative PCR method and to check the cytokine level, we use the ELISA method. Result: The frequency of Treg cells, followed by the expression of transcription factor Foxp3, was greatly reduced in these patients compared to the control group (p=0.0002) Interestingly, in relation to the expression and concentration of TGF-β, in both PBMC, decrease was observed compared to the control group (p<0.0001). The results of the investigation of microRNA related to Treg cells also showed an increase in the expression of miRNA-155 (p=0.0002).